In-vitro 60Co dose calibration curve using dicentrics assay technique for the Malaysian National Biodosimetry Laboratory

Publication Issue: 
Volume 31, Number 1-4, 2010
Page No: 
Date Received: 
Tuesday, April 20, 2010
Authors' Name: 
Noriah Jamal
Rahimah Abdul Rahim
Noraisyah Yusof
Juliana Mahamad Napiah
Nelly Bo Nai Lee
Yahaya Talib
Rehir Dahalan
Authors' Affiliation and Address: 
Medical Technology Division, Malaysian Nuclear Agency, 43000 Kajang, Malaysia

Ionizing radiation is a strong clastogen, causing
chromosome breakage, and resulting in cytogenetic
aberrations in exposed cells. Accurate estimation of the
level of absorbed dose is important immediately after
exposure as a guide for medical treatment and at longer
times after exposure to assess the possible health
consequences. Cytogenetic analysis of peripheral blood
lymphocytes can provide a biological estimation of the
dose received in exposure to ionizing radiation [1]. A
number of cytogenetic assay techniques have been
developed for dose estimation, including dicentric,
Fluorescence In–Situ Hybridization (FISH), micronuclei
and Premature Chromosome Condensation (PCC) [2,3].
Dicentric is the aberration type that is most
frequently used in biological dosimetry. This is because
it clearly involves an interaction (or exchange) between
two chromosomes. This assay technique is generally
accepted as the most specific, sensitive and currently
available method for determining doses from recent (i.e.
within days to about 6 months) exposure to ionizing
radiation [4]. It could be especially useful in providing
evidence of non-uniform exposure and conformation of
individuals in a high dose exposure triage category, and
the most sensitive method of quantifying the radiation
dose in the absence of physical measurements due to its
ability to estimate the average whole-body dose [2]. It is
a reliable and useful tool in medical management of
radiation accident victims [4].

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